The cell-surface proteome plays a critical role in immune-cell function; however, our ability to examine its interactions and spatial organization has previously been limited by available proteomic techniques. This episode explores the function of immune-cell membrane proteins and how the latest developments in spatial proteomics have enabled more detailed interrogation of these proteins and their spatial relationships.Our guest, Hanna van Ooijen, Immunology Application Scientist at Pixelgen Technologies guides us through the field, revealing a new technique that enables spatial analysis of the cell-surface proteome at a single-cell resolution and highlighting some exciting discoveries that it has facilitated. <h2>Contents:</h2><ul><li>Introductions: 00:00-01:40</li><li>Introducing Molecular Pixelation: 01:40-02:15</li><li>Example applications of Molecular Pixelation: 02:15-03:20</li><li>The role of membrane proteins in immune cell function: 03:20-07:25</li><li>Traditional techniques to investigate cell membrane proteins: 07:15-10:20</li><li>Recent improvements in investigative technology and our understanding of immunology: 10:20-11:10</li><li>Challenges associated with current technologies: 11:10-13:50</li><li>How Molecular Pixelation can address these challenges: 13:50-15:25</li><li>Molecular Pixelation workflow: 15:25-17:55</li><li>Tips for best practice when using molecular pixelation: 17:55-19:30</li><li>Exciting discoveries using Molecular pixelations: 19:30-21:00</li><li>Potential implications of molecular pixelation for the future of immunology: 21:00-24:00</li></ul>

Spatial analysis of the immune-cell-surface proteome at a single-cell resolution 

In this episode of Talking Techniques, we catch up with Michael Long, Principle Investigator of the <a href="https://longlab.med.nyu.edu/research/" rel="noopener noreferrer" target="_blank">Long Lab</a> at New York University (NY, USA), where he investigates the neural circuits that underlie vocal communication.Through the examination of animal models, from songbirds to the rare singing mice of Costa Rica, with cutting-edge imaging techniques Michael reveals fascinating insights into vocal communication. We also discuss his human experiments, working alongside neurosurgeons, with emerging electrophysiological probes to monitor the neural activity of participants as they speak and interact, ultimately revealing how this research could begin to provide solutions for neurological conditions impacting communication, such as autism.<h2> </h2><h2>Contents:</h2><ul><li>Introduction: 00:00 – 01:40</li><li>Investigating neural circuits underlying vocal communication: 01:40 – 04:15</li><li>Techniques to explore animal models of vocal communication: 04:15 – 06:25</li><li>The impact of cooling brain regions on songbird singing: 06:25 – 07:50</li><li>The techniques used to investigate animal models:&nbsp;07:50 – 12:20</li><li>Songbirds: 07:50 – 09:45</li><li>The singing mouse: 10:00 – 12:20</li><li>Investigating neural circuits in humans during speech: 12:20 – 16:30</li><li>Investigating neural circuits in humans during conversation: 16:30 – 19:00</li><li>Moving beyond neural area identification towards understanding neural pathways and mechanisms: 19:00 – 21:40</li><li>Navigating neuropixels, big data and safety: 21:40 – 26:10</li><li>If there was one thing you could ask for to help you better understand these pathways, what would it be? 26:10 – 27:55</li><li>The experience of working with patients undergoing neurosurgery: 27:55 – 30:30</li><li>The potential impact on speech disorders and autism: 30:30 – 33:15</li></ul>

Investigating the neurological pathways underlying vocal communication

Launching our fourth season of Talking Techniques, this episode, supported by the University of Cincinnati (OH, USA) we delve into rare disease research and pharmacogenomics, their intersection and the key techniques used to explore them.Guiding us through these fields is Brenna Carey, an Assistant Professor at Cincinnati Children’s Hospital Medical Center whose research focuses on rare disease pathogenesis, diagnostics and therapeutic development and who also runs key courses on the University’s <a href="https://online.uc.edu/masters-programs/ms-pharmaceutical-sciences-pharmacogenomics-and-personalized-healthcare/?utm_source=response-solutions&amp;utm_medium=video&amp;utm_campaign=ms-pgx-1-24-24-podcast&amp;utm_content=biotechniques" rel="noopener noreferrer" target="_blank">Pharmacogenomics</a> and <a href="https://online.uc.edu/masters-programs/ms-pharmaceutical-science-in-drug-development/?utm_source=response-solutions&amp;utm_medium=video&amp;utm_campaign=ms-dd-1-24-24-podcast&amp;utm_content=biotechniques" rel="noopener noreferrer" target="_blank">Drug Discovery</a> Masters degree programs. Contents:Introduction: 00:00-01:15An introduction to pulmonary alveolar proteinosis (PAP) and rare lung diseases 01:15-03:50Techniques to investigate the pathogenesis of PAP: 03:50-05:30Developing diagnostics and therapeutics for PAP: 05:30-08:20The importance of pharmacogenomics in drug development: 08:20-11:25Key techniques and approaches in pharmacogenomics: 11:25-13:00Emerging trends in pharmacogenomics: 13:00-15:05Key takeaways from your pharmacogenomics course: 15:00-18:00What would you ask for to improve our understanding of pharmacogenomics? 18:00-20:15 This episode is supported by the <a href="https://online.uc.edu/" rel="noopener noreferrer" target="_blank">University of Cincinnati Online</a><a href="https://online.uc.edu/" rel="noopener noreferrer" target="_blank"><img src="https://www.biotechniques.com/wp-content/uploads/2024/01/171939_171932_Keystone-Logo.png"></a>

Rare disease and pharmacogenomics

In this episode of Talking Techniques, we talk to Andrew Lee, a senior research fellow in Queen’s University Belfast’s (UK) wastewater-based epidemiology group, about his work using wastewater to monitor and detect infectious diseases. Andrew discusses how wastewater surveillance acts as an early warning system, providing novel, unbiased insights into human and animal pathogens that are circulating within a community, and how this can contribute to a ‘One Health’ approach. He also explains how he has incorporated nanopore sequencing into his work, and the advantages that this provides. <h2>Contents:</h2>·&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;00:00­–01:45: Introductions·&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;01:45–03:45: Wastewater surveillance for infectious disease·&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;03:45–05:35: Genomic surveillance approaches can complement established epidemiological methods·&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;05:35–07:25: Why look at wastewater?·&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;07:25–10:40: The advantages of nanopore sequencing for wastewater surveillance·&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;10:40–12:25: The experimental workflow·&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;12:25–15:05: Using wastewater surveillance to detect both human and avian influenza·&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;15:05–18:20: Wastewater surveillance as an early warning system·&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;18:20–20:47: Future perspectives: other environmental samples, antimicrobial resistance and what else can be found in wastewater?

One man’s waste in another man’s treasure: using wastewater to monitor infectious diseases

In this episode of Talking Techniques, we speak to two experts from Sino Biological US Inc. (PA, USA) about the latest developments in antibody technologies and how these developments have led to the next generation of antibodies that are revolutionizing therapeutic approaches to a number of diseases.With the guidance of Field Scientist Ritwika Biswas and Technical Account Manager Grace Liu, we explore the challenges of developing and working with next-generation antibodies, the latest developments and applications of these molecules and the holy grail that antibody designers are driving towards. Contents:<ul><li>Introduction: 00:00 – 02:40</li><li>The history of monoclonal antibody therapeutics: 02:40 – 04:40</li><li>The working principles of multi-specific antibodies: 04:40 – 08:15</li><li>Recent developments in ADCs: 08:15 – 11:35</li><li>Challenges with the development of multi-specific antibodies and ADCs: 11:35 – 13:55</li><li>Solutions to address these challenges: 13:55 – 16:25</li><li>Clinical applications of multi-specific antibodies and ADCs: 16:25 – 20:30</li><li>The dream of real-time adaptability for the next generation of antibody therapeutics: 20:30 – 24:52</li></ul>

Next-generation antibody therapeutics

In this episode of Talking Techniques, Rachel Thijssen, an Assistant Professor at Amsterdam University Medical Centers (UMC; the Netherlands), discusses her research using single-cell analyses to investigate treatment-resistant leukemia cells. Rachel explains her single-cell technique called rapid capture hybridization sequencing (RaCH-seq), how she utilizes nanopore sequencing, and how she hopes that this technique can be used to gain new insights into disease and improve therapies. &nbsp;<h2>Contents:</h2>00:00–01:35: Introduction 01:35–03:25: Single-cell sequencing in leukemia research03:25–05:15: What is single-cell RaCH-seq?05:15–06:10: Using nanopore sequencing for RaCH-seq06:10–07:30: How can other researchers apply RaCH-seq to their work?07:30–09:50: Looking to the future: spatial biology, collaborations and improved therapies&nbsp;

Investigating resistant leukemia with single-cell technologies 

In this episode of Talking Techniques, Ritwika Biswas, Field Scientist at Sino Biological US Inc. (PA, USA), walks us through the chimeric antigen receptor (CAR) development process and how T and natural killer (NK) cells can be utilized to carry these modular synthetic molecules. Ritwika also addresses the safety and efficacy of these cell therapies as well as the ethical considerations around them.

CAR-T and CAR-NK cell therapies

In this episode, supported by Fortis Life Sciences, we delve into the relationship between mitochondria, inflammation and cancer, discussing the new techniques that are bearing fruit in this field, such as spatial analysis.Our expert insight for this episode comes from Phillip West, Principle Investigator of the West lab at Texas A&amp;M Medicine (TX, USA). Philip explains the role mitochondria can play in cancer and heart disease, reveals some of his most exciting discoveries of late and provides technical tips for investigating this field.Listen on to discover how his use of spatial techniques has helped uncover mechanisms linking mitochondrial damage to the stifling of the immune system in the tumor microenvironment and the latest breakthroughs at the intersection of mitochondria and cancer.Contents:<ul><li>Introduction: 00:00-01:30</li><li>The innate immune system, in inflammation and disease: 01:30-04:20</li><li>The role of mitochondria in innate immunity: 04:20-07:00</li><li>Categorizing DAMPS and their role in cardiovascular disease: 07:00-08:40</li><li>Mitochondria and cancer: 08:40-11:55</li><li>Techniques for the investigation of mitochondria: 11:55-15:20</li><li>Best practice techniques for spatial studies: 15:20-17:35</li><li>Discoveries made using spatial approaches to mitochondrial investigations: 17:35-19:55</li><li>The latest developments in the intersection of mitochondria, cancer and inflammation: 19:55-22:20</li><li>What is one thing you would ask for to improve your understanding of this field? 22:20-24:20</li></ul>

Mitochondria, the immune system and cancer: discovering new insights with spatial technologies

In this episode, sponsored by Zymo Research, we explore the microbiome and how long-read sequencing techniques are changing our approach to the field and the exciting discoveries that they have led to. To do this we speak to Kris Locken, Molecular Biologist in the Research &amp; Development department of Zymo Research (CA, USA), and Jeremy Wilkinson, Global Marketing Specialist for Microbial Genomics at PacBio (CA, USA). Find out how microbes compare to nuts and what this means for the challenges of metagenomic sample preparation, how long reads can stack up vs short reads for metagenome assembly and much more, all in this latest episode of Talking Techniques <h2>Contents:</h2>Intro: 00:00-01:55What is metagenomics and why is it important for microbial studies? 01:55-03:55How has long-read sequencing impacted metagenomics? 03:55-06:05Long-read vs short-read sequencing workflows: 06:05-07:15Addressing barriers to long-read sequencing: 07:15-08:45Sample preparation for long-read sequencing: 08:45-12:20Development of long-read sequencing to improve accuracy and capabilities: 12:20-14:40Best practice for assembly and analysis: 14:40-17:40Exciting examples of the benefits of long-read sequencing: 17:40-20:00What would you wish for to improve the ability long-read sequencing in microbiomics: 20:00-21:40

Long-read sequencing, metagenomics and the microbiome

The latest in lab updates and life science developments

Welcome to Talking Techniques! In this Podcast BioTechniques Digital Editor Tristan Free, interviews researchers at the forefront of their fields about the latest breakthroughs, controversies and conversations in the life sciences. From CRISPR to COVID-19, organoids to the microbiome, this podcast will explore the latest developments in the lab and interesting applications of techniques, while trying to determine how we can drive science forward in progressive and inventive ways.

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