Talking Techniques


rAAVs, host-cell contamination and ddPCR

Season 3, Ep. 10

In this episode of Talking Techniques, supported by Bio-Rad, we discuss a key component of many gene therapies: recombinant adeno-associated viruses (rAAVs) and their production. These viruses act as efficient, accurate delivery vesicles for the gene therapy’s plasmid.

Speaking to Associate Director of Biopharma Product Marketing at Bio-Rad Laboratories, Mark White, we take a look at the different expression systems used for their production and compare their advantages, before looking at some of the challenges involved in the production rAAVs, such as host-cell contamination.

Discover the tools that can help minimize host-cell contamination and differentiate between nuclease resistant and nuclease reactive contaminant DNA and find out about some of the most exciting developments in rAAV technologies. 

  • The role of rAAVs in gene therapies: 00:40-02:15
  • The production of rAAVs and gene therapies: 02:15-03:30
  • Why are HEK cells so popular for cell therapy production? 03:30-05:45
  • HEK vs SF9 Insect cell expression systems: 05:45-06:45
  • Challenges in cell therapy expression systems: 06:45-08:05
  • Host DNA contamination: 08:05-10:30
  • The risks of host DNA contamination: 10:30-12:45
  • Key techniques to minimize host DNA contamination: 12:45-14:40
  • The advantages of ddPCR in gene therapy production: 14:40-17:50
  • Distinguishing between nuclease resistant and nuclease reactive host cell DNA: 17:50-19:10
  • The most exciting developments in rAAV technology: 19:10-20:20
  • What is one thing you would ask for to improve rAAV and gene therapy development 20:20-26:38

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