Lifescience Knowledge Center

Advances in massive parallel DNA sequencing technologies have enabled RNA-seq by sequencing of cDNA. RNA-seq is a revolutionary tool with better resolution and higher reproducibility that offers many advantages over the traditional microarray technologies. RNA-seq can be used to extend our knowledge of alternative splicing events, novel genes and transcripts, and fusion transcripts. RNA-seq can also help to understand molecular mechanisms and then it tells about signaling pathways which controls embryonic development. The transcriptome data give researchers a good place to start searching for a newly found gene's function.

RNA-seq is a revolutionary tool with better resolution and higher reproducibility that offers many advantages over the traditional microarray technologies. RNA-seq can be used to extend our knowledge of alternative splicing events, novel genes and transcripts, and fusion transcripts. RNA-seq can also help to understand molecular mechanisms and then it tells about signaling pathways which controls embryonic development. The transcriptome data give researchers a good place to start searching for a newly found gene's function.

The transcriptome is the entire set of RNA transcripts in a given cell for a specific developmental stage or physiological condition. RNA is a polymeric molecule implicated in various biological processes, such as the coding, decoding, regulation, and expression of genes. RNA molecules include mRNAs, long non-coding RNA (lncRNAs), circular RNA (circRNAs), microRNA (miRNA), message RNA (mRNA), transfer (tRNA) and ribosomal ribonucleic acid (rRNA).

RNA-seq is an important tool for interpreting the functional elements of the genome as well as for understanding the underlying mechanisms of diverse diseases, especially cancers.

The transcriptome is the entire set of RNA transcripts in a given cell for a specific developmental stage or physiological condition. RNA is a polymeric molecule implicated in various biological processes, such as the coding, decoding, regulation, and expression of genes. RNA molecules include mRNAs, long non-coding RNA (lncRNAs), circular RNA (circRNAs), microRNA (miRNA), message RNA (mRNA), transfer (tRNA) and ribosomal ribonucleic acid (rRNA).

Whole transcriptome sequencing (WTS) is used to reveal the presence and quantity of RNA, in a biological sample under specific conditions.