{"version":"1.0","type":"rich","provider_name":"Acast","provider_url":"https://acast.com","height":250,"width":700,"html":"<iframe src=\"https://embed.acast.com/$/5f2442bc6de29f32c4d05451/65e85ce6b75ceb0016bd2f70?\" frameBorder=\"0\" width=\"700\" height=\"250\"></iframe>","title":"Spatial analysis of the immune-cell-surface proteome at a single-cell resolution ","thumbnail_width":200,"thumbnail_height":200,"thumbnail_url":"https://open-images.acast.com/shows/5f2442bc6de29f32c4d05451/1710953949907-4d30075c1848164cb37925e3c36b1b25.jpeg?height=200","description":"<p>The cell-surface proteome plays a critical role in immune-cell function; however, our ability to examine its interactions and spatial organization has previously been limited by available proteomic techniques. This episode explores the function of immune-cell membrane proteins and how the latest developments in spatial proteomics have enabled more detailed interrogation of these proteins and their spatial relationships.</p><p>Our guest, Hanna van Ooijen, Immunology Application Scientist at Pixelgen Technologies guides us through the field, revealing a new technique that enables spatial analysis of the cell-surface proteome at a single-cell resolution and highlighting some exciting discoveries that it has facilitated.</p><p><br></p><h2>Contents:</h2><ul><li>Introductions: 00:00-01:40</li><li>Introducing Molecular Pixelation: 01:40-02:15</li><li>Example applications of Molecular Pixelation: 02:15-03:20</li><li>The role of membrane proteins in immune cell function: 03:20-07:25</li><li>Traditional techniques to investigate cell membrane proteins: 07:15-10:20</li><li>Recent improvements in investigative technology and our understanding of immunology: 10:20-11:10</li><li>Challenges associated with current technologies: 11:10-13:50</li><li>How Molecular Pixelation can address these challenges: 13:50-15:25</li><li>Molecular Pixelation workflow: 15:25-17:55</li><li>Tips for best practice when using molecular pixelation: 17:55-19:30</li><li>Exciting discoveries using Molecular pixelations: 19:30-21:00</li><li>Potential implications of molecular pixelation for the future of immunology: 21:00-24:00</li></ul>","author_name":"BioTechniques"}